UV-induced Spectral Shift and Protonation of DNA Fluorescent Dye Hoechst 33258
نویسندگان
چکیده
منابع مشابه
Measurement of cell numbers in microtiter culture plates using the fluorescent dye Hoechst 33258.
A sensitive fluorimetric assay based on DNA binding of the fluorescent dye Hoechst 33258 was developed to quantitate cell numbers in microtiter plates. The cells were fixed and incubated with the dye solution. The bound dye was extracted with ethanol and fluorescence was measured in a spectrofluorimeter. The method is sensitive for as little as a thousand cells and is particularly useful for th...
متن کاملQuantitation of DNA using Hoechst 33258
An essential element of cellular and molecular biology is the ability to quantitate DNA in large numbers of samples at a sensitivity that enables determination of small amounts of sample. Here we describe a fluorescent method to quantitate DNA with Hoechst 33258 dye and the BioTek FL600 fluorescence microplate reader. This assay provides linear results over three orders of magnitude and can rou...
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The Hoechst dye fluorescence technique, which is used for the quantitative determination of DNA (12), requires high saline concentrations at neutral pH to reach maximal fluorescence of the DNA-dye complex and to render the DNA fully accessible to the dye by dissociating foreign molecules (3,12). In conventional assays, the DNA is alcohol-precipitated and then resuspended in buffer containing 2 ...
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We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of compact-globular and hollow-toroidal particles. In order to explore the functio...
متن کاملBinding Mechanisms of Methylene Blue and Hoechst 33258 with Dna
In this work the data of methylene blue (MB) and Hoechst 33258 interaction with DNA are presented. It has been shown that at 0.002 M ionic strength of solution MB is bound to double-stranded DNA by three modes: intercalation, semi-intercalation and electrostatic. It was revealed that at higher ionic strengths of solution complete intercalation of MB becomes thermodynamically unacceptable. It wa...
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ژورنال
عنوان ژورنال: Journal of Fluorescence
سال: 2014
ISSN: 1053-0509,1573-4994
DOI: 10.1007/s10895-014-1468-y